Pan Cytokeratin Antibody Cocktail (Acidic + Basic) orb386219 from Biorbyt

Description

Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody detects acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins: 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. It is a broad spectrum anti pan-cytokeratin antibody, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It may be useful to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has high sensitivity in the recognition of epithelial cells and carcinomas

Product Specs
ItemPan Cytokeratin Antibody Cocktail (Acidic + Basic)
CompanyBiorbyt
Price Pricing Info Supplier Page
Catalog Numberorb386219
Quantity20 µg, 100 µg
ApplicationsFACS, IF, IHC-P, WB
Conjugate/TagUnconjugated
PurityProtein A affinity chromatography
ReactivityHuman
TargetPan Cytokeratin
IsotypeRabbit IgG
HostRabbit
ImmunogenRecombinant full-length human KRT76 and KRT77 proteins were used as the immunogen for this pan Cytokeratin antibody
NCBI Full Gene Namekeratin 5, keratin 4, alanyl-tRNA synthetase 1, keratin 1, keratin 3, keratin 8, keratin 19, N-acetyltransferase 2, ATP binding cassette subfamily A member 1, aralkylamine N-acetyltransferase, keratin 15, keratin 14, keratin 16, keratin 10, angio associated migratory cell protein, keratin 6A
NCBI Gene AliasesDEE29, EBS2F, EBS2E, EBS2D, EBS2C, EBS2B, EBS2A, CK-6C, CK-6E, CK-4, CK-8, EBS1B, EBS1C, EBS1A, EBS1D, NEPPK, EHK, CK6D, CK5, CK4, CK1, IHL, SNAT, CK8, CK14, CK16, KRT1A, K4, AEI2, EHK2, EHK1, CARD2, KPP, MECD2, NFJ, BCIE, DDD, PNAT, FNEPPK, PC3, EBS2, EBS3, EBS4, CK3, K10, K16, K15, K14, TTD8, K19, EBS1, K3, CK10, K1, CK15, CK6A, K5, CK6C, CK19, K8, EPPK, KRT6D, KRT6C, K6D, K6C, K6A, K1CP, K1CS, K1CO, KO, CMT2N, ABC1, HDLCQTL13, HPALP1, PC1, NAT-2, WSN1, K2C8, KRT16A, DDD1, HDLS2, BIE, ABC-1, TGD, CYK4, CYK8, HDLDT1, EIEE29, AARS, DSPS, KRT5A, CERP, AAC2
ClonalityPolyclonal
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
StorageStore the pan Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide)

Specifications/Features

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the pan Cytokeratin antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Application Dilutions: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 0.5-1ug/ml for 2 hours at RT,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT

Add to Compare List